The Allen Institute has generated a variety of human embryonic stem cell lines that report the expression of selected genes, or cause the disruption of target genes. Selected lines are available through WiCell's Allen Institute Stem Cell Collection for Early Human Brain Development. Other lines, as well as vectors and plasmid maps, are available by request.
Cell line
Parent cell line
Target gene (alias)
Line genotype
Engineered change
Reference
Request information
Comments
AI01e-SOX2OCT4
H1 (WA01)
SOX2; POU5F1 (OCT4)
SOX2+/Citrine; POU5F1+/tdTomato
SOX2-Flag-Citrine-P2A-PuroR; POU5F1-HA-tdTomato-IRES-NeoR-pA
Nuclear fluorescence of both markers observed in undifferentiated hESCs
AI03e-DCXYFP
H1 (WA01)
DCX
DCXCitrine/Y
DCX-Citrine-Flag
Non-nuclear fluorescence only after neuronal differentiation into neurons
AI04e-FOXG1YFP
H1 (WA01)
FOXG1
FOXG1+/Citrine
FOXG1-P2A-Citrine-pA
Citrine fluorescence only after differentation but neural differentiation appeared abnormal compared to parental line
AI05e-OCT4RFP
H1 (WA01)
POU5F1 (OCT4)
POU5F1+/tdTomato
POU5F1-tdTomato-IRES-Puro-pA
Nuclear fluorescence observed in undifferentiated hESCs
AI06e-SOX2YFP
H1 (WA01)
SOX2
SOX2+/Citrine
SOX2-Flag-Citrine-P2A-PuroR
Nuclear fluorescence observed in undifferentiated hESCs and in progenitors after neural differentiation
AI07e-Timothy
H1 (WA01)
CACNA1C
CACNA1CG406R/G406R
CACNA1CG406R/G406R
No fluorescent marking
AI08e-PAX6YFP
H1 (WA01)
PAX6
PAX6+/Citrine
PAX6-P2A-Citrine-pA
Dim nuclear fluorescence observed in neural progenitors after differentiation
AI09e-KCTD13a
H1 (WA01)
KCTD13
KCTD13+/-
KCTD13 disruption
No fluorescent marking
AI10e-KCTD13b
H1 (WA01)
KCTD13
KCTD13+/-
KCTD13 disruption
No fluorescent marking
AI11e-OTX2YFP
H1 (WA01)
OTX2
OTX2+/Citrine
OTX2-Flag-Citrine-P2A-PuroR
Nuclear fluorescence after neural differentiation into neural progenitors
AI12e-HOPX-CIT+/-
H1 (WA01)
HOPX
HOPX+/Citrine
HOPX-Flag-Citrine
Fluorescence after neural differentiation into progenitors and glia
AI13e-HOPX-CIT+/+
H1 (WA01)
HOPX
HOPX+/Citrine
HOPX-Flag-Citrine
Fluorescence after neural differentiation into progenitors and glia
mOTX2-YFP
G4 (129S6 x B6 F1 hybrid)
mouse Otx2
mOtx2+/Citrine
mOtx2-Citrine-pA
Nuclear fluorescence after neural differentiation into neural progenitors
