Viral transduction is often used in a molecular toolkit to modify neurons; the Allen Institute for Brain Science uses viruses to target and label specific classes of neurons for further study. A variety of different viruses have been generated and are available to the community. This expanding list highlights viral reagents that have been used in recently published studies.
Name
Type of virus
Promoter used
Expression control
Transgene expressed
Function
Plasmid info
Comments
AAV pCAG-FLEX2-tTA2-WPRE-bGHpA
AAV
CAG
Cre-dependent driver
tTA2
Transcriptional activation
AAV pCAG-FLEX-EGFP-WPRE
AAV
CAG
Cre-dependent driver
EGFP
Fluorescent labeling
AAV pCAG-FLEX-tdTomato-WPRE
AAV
CAG
Cre-dependent driver
tdTomato
Fluorescent labeling
AAV pCAG-FSF-FLEX-EGFP-WPRE-bGHpA
AAV
CAG
Cre and Flp dependent
EGFP
Fluorescent labeling
AAV pCAG-LSL-tdTomato
AAV
CAG
Cre-dependent driver
tdTomato
Fluorescent labeling
AAV phSyn1(S)-DreO-bGHpA
AAV
Human Syn1
Driver
Mammalianized Dre
Recombination
AAV phSyn1(S)-FLEX-EGFP-WPRE
AAV
Human Syn1
Cre-dependent driver
EGFP
Fluorescent labeling
AAV phSyn1(S)-FLEX-tdTomato-T2A-SypEGFP-WPRE
AAV
Human Syn1
Cre-dependent driver
tdTomato-T2A-SypEGFP
Synaptic flourescent labeling
AAV phSyn1(S)-FLEX-tdTomato-WPRE
AAV
Human Syn1
Cre-dependent driver
tdTomato
Fluorescent labeling
AAV phSyn1(S)-FlpE-bGHpA
AAV
Human Syn1
Driver
Enhanced Flp
Recombination
AAV phSyn1(S)-FlpO-bGHpA
AAV
Human Syn1
Driver
Mammalianized Flp
Recombination
AAV phSyn1(S)-tdTomato-WPRE
AAV
Human Syn1
Driver
tdTomato
Fluorescent labeling
AAV phSyn1-FSF-FLEX-ChR2(H134R)-EYFP-WPRE-bGHpA
AAV
Human Syn1
Cre and Flp dependent
ChR2(H134R)-EYFP
Optogenetic activator
AAV phSyn1-RSR-FLEX-ChR2(H134R)-EYFP-WPRE-bGHpA
AAV
Human Syn1
Cre and Flp dependent
ChR2(H134R)-EYFP
Optogenetic activator
AAV pTRE-FLEX-EGFP-WPRE-bGHpA
AAV
TRE
Cre and tTa dependent
EGFP
Fluorescent labeling
AAV pTRE-FSF-FLEX-EGFP-WPRE-bGHpA
AAV
TRE
Cre, Flp, and tTa dependent
EGFP
Fluorescent labeling
rAAV-mscRE16-minBGpromoter-EGFP-WPRE-hGHpA
AAV
Beta Globin minimal promoter
Enhancer dependent driver
EGFP
Fluorescent labeling
rAAV-mscRE4-minBGpromoter-iCre-WPRE-hGHpA
AAV
Beta Globin minimal promoter
Enhancer dependent driver
iCre
Recombination
rAAV-mscRE4-minBGpromoter-tTA2-WPRE-hGHpA
AAV
Beta Globin minimal promoter
Enhancer dependent driver
tTA2
Transcriptional activation
rAAV-mscRE10-minBGpromoter-FlpO-WPRE-hGHpA
AAV
Beta Globin minimal promoter
Enhancer dependent driver
FlpO
Recombination
rAAV-mscRE13-minBGpromoter-FlpO-WPRE-hGHpA
AAV
Beta Globin minimal promoter
Enhancer dependent driver
FlpO
Recombination
rAAV-mscRE16-minBGpromoter-FlpO-WPRE-hGHpA
AAV
Beta Globin minimal promoter
Enhancer dependent driver
FlpO
Recombination
rAAV-mscRE10-minBGpromoter-iCre-WPRE-hGHpA
AAV
Beta Globin minimal promoter
Enhancer dependent driver
iCre
Recombination
rAAV-mscRE13-minBGpromoter-iCre-WPRE-hGHpA
AAV
Beta Globin minimal promoter
Enhancer dependent driver
iCre
Recombination
rAAV-mscRE16-minBGpromoter-iCre-WPRE-hGHpA
AAV
Beta Globin minimal promoter
Enhancer dependent driver
iCre
Recombination
rAAV-mscRE10-minBGpromoter-tTA2-WPRE-hGHpA
AAV
Beta Globin minimal promoter
Enhancer dependent driver
tTA2
Transcriptional activation
rAAV-mscRE13-minBGpromoter-tTA2-WPRE-hGHpA
AAV
Beta Globin minimal promoter
Enhancer dependent driver
tTA2
Transcriptional activation
rAAV-mscRE16-minBGpromoter-tTA2-WPRE-hGHpA
AAV
Beta Globin minimal promoter
Enhancer dependent driver
tTA2
Transcriptional activation
rAAV-Synapsin promoter-oNigri-WPRE-bGHpA
AAV
Synapsin promoter
Enhancer dependent driver
oNigri
Recombination
rAAV-mscRE16-minBGpromoter-oNigri-WPRE-hGHpA
AAV
Beta Globin minimal promoter
Enhancer dependent driver
oNigri
Recombination
scAAV-mscRE4-minBGpromoter-SYFP2-WPRE3-bGHpA
AAV
Beta Globin minimal promoter
Enhancer dependent driver
SYFP2
Flourescent labeling
rAAV-mscRE4-minBGpromoter-FlpO-WPRE-hGHpA
AAV
Beta Globin minimal promoter
Enhancer dependent driver
FlpO
Recombination
rAAV-mscRE4-minBGpromoter-EGFP-WPRE-hGHpA
AAV
Beta Globin minimal promoter
Enhancer dependent driver
EGFP
Flourescent labeling
rAAV-mscRE10-minBGpromoter-EGFP-WPRE-hGHpA
AAV
Beta Globin minimal promoter
Enhancer dependent driver
EGFP
Flourescent labeling
CN1203-scAAV-hDLXI56i-minBG-SYFP2-WPRE3-BGHpA
AAV
minBetaGlobin
Enhancer dependent driver
SYFP2
Flourescent labeling
CN1244-rAAV-hDLXI56i-minBG-SYFP2-WPRE3-BGHpA
AAV
minBetaGlobin
Enhancer dependent driver
SYFP2
Flourescent labeling
CN1253-scAAV-eHGT_017h-minBG-SYFP2-WPRE3-BGHpA
AAV
minBetaGlobin
Enhancer dependent driver
SYFP2
Flourescent labeling
CN1255-scAAV-eHGT_019h-minBG-SYFP2-WPRE3-BGHpA
AAV
minBetaGlobin
Enhancer dependent driver
SYFP2
Flourescent labeling
CN1258-scAAV-eHGT_022h-minBG-SYFP2-WPRE3-BGHpA
AAV
minBetaGlobin
Enhancer dependent driver
SYFP2
Flourescent labeling
CN1259-scAAV-eHGT_023h-minBG-SYFP2-WPRE3-BGHpA
AAV
minBetaGlobin
Enhancer dependent driver
SYFP2
Flourescent labeling
CN1279-scAAV-eHGT_022m-minBG-SYFP2-WPRE3-BGHpA
AAV
minBetaGlobin
Enhancer dependent driver
SYFP2
Flourescent labeling
CN1294-scAAV-hI56i-minBglobin-iCre-WPRE3-BGHpA
AAV
minBetaGlobin
Enhancer dependent driver
iCre
Recombination
CN1390-rAAV-3x(hDLXI56i-3xCore)-minBG-SYFP2-WPRE3-BGHpA
AAV
minBetaGlobin
Enhancer dependent driver
SYFP2
Flourescent labeling
CN1402-rAAV-eHGT_058h-minBG-SYFP2-WPRE3-BGHpA
AAV
minBetaGlobin
Enhancer dependent driver
SYFP2
Flourescent labeling
CN1408-rAAV-eHGT_064h-minBG-SYFP2-WPRE3-BGHpA
AAV
minBetaGlobin
Enhancer dependent driver
SYFP2
Flourescent labeling
CN1457-rAAV-eHGT_078h-minBG-SYFP2-WPRE3-BGHpA
AAV
minBetaGlobin
Enhancer dependent driver
SYFP2
Flourescent labeling
CN1466-rAAV-eHGT_078m-minBG-SYFP2-WPRE3-BGHpA
AAV
minBetaGlobin
Enhancer dependent driver
SYFP2
Flourescent labeling
CN1496-rAAV-hDlxI56i-minBglobin-H2B-nsSYFP2-WPRE3-BGHpA
AAV
minBetaGlobin
Enhancer dependent driver
H2B-SYFP2
Flourescent labeling
CN1525-rAAV-hsA2-eHGT_079h-minRho-SYFP2-WPRE3-BGHpA
AAV
MinRho
Enhancer dependent driver
SYFP2
Flourescent labeling
CN1528-rAAV-hsA2-eHGT_082h-minRho-SYFP2-WPRE3-BGHpA
AAV
MinRho
Enhancer dependent driver
SYFP2
Flourescent labeling
CN1621-rAAV-hsA2-eHGT_128h-minRho-SYFP2-WPRE3-BGHpA
AAV
MinRho
Enhancer dependent driver
SYFP2
Flourescent labeling
CN1633-rAAV-hsA2-eHGT_140h-minRho-SYFP2-WPRE3-BGHpA
AAV
MinRho
Enhancer dependent driver
SYFP2
Flourescent labeling
CN1818-rAAV-3x(core)mscRE4-minCMV-SYFP2-WPRE3-BGHpA
AAV
minCMV
Enhancer dependent driver
SYFP2
Flourescent labeling
CN1839-rAAV-hSyn1-SYFP2-10aa-H2B-WPRE3-BGHpA
AAV
hSyn1
Driver
H2B-SYFP2
Flourescent labeling
CN1851-rAAV-hI56i-minBglobin-iCre-4X2C-WPRE3-BGHpA
AAV
minBetaGlobin
Enhancer dependent driver
iCre
Recombination
CN2014-rAAV-mscRE4-minCMV-SYFP2-WPRE3-BGHpA
AAV
minCMV
Enhancer dependent driver
SYFP2
Flourescent labeling
CN2045-rAAV-3xSP10ins-eHGT_359h-minRho*-SYFP2-WPRE3-BGHpA
AAV
MinRho
Enhancer dependent driver
SYFP2
Flourescent labeling
CN2249-rAAV-eHGT_451m-minBglobin-SYFP2-WPRE3-BGHpA
AAV
minBetaGlobin
Enhancer dependent driver
SYFP2
Flourescent labeling
CN2251-rAAV-eHGT_453m-minBglobin-SYFP2-WPRE3-BGHpA
AAV
minBetaGlobin
Enhancer dependent driver
SYFP2
Flourescent labeling
CN2254-rAAV-eHGT_459m-minBglobin-SYFP2-WPRE3-BGHpA
AAV
minBetaGlobin
Enhancer dependent driver
SYFP2
Flourescent labeling
CN2255-rAAV-eHGT_460m-minBglobin-SYFP2-WPRE3-BGHpA
AAV
minBetaGlobin
Enhancer dependent driver
SYFP2
Flourescent labeling
CN2256-rAAV-eHGT_462m-minBglobin-SYFP2-WPRE3-BGHpA
AAV
minBetaGlobin
Enhancer dependent driver
SYFP2
Flourescent labeling
Research reported in this publication was supported by the National Institute Of Mental Health of the National Institutes of Health under Award Number RF1MH114126. The content is solely the responsibility of the authors and does not necessarily represent the official views of the National Institutes of Health.
Research reported in this publication was supported by the National Institute Of Mental Health of the National Institutes of Health under Award Number RF1MH121274. The content is solely the responsibility of the authors and does not necessarily represent the official views of the National Institutes of Health.
The project described was supported by award number R01DA036909 from the Office of the Director and the National Institute on Drug Abuse. Its contents are solely the responsibility of the authors and do not necessarily represent the official views of the National Institutes of Health and the National Institute on Drug Abuse.
