Human Multiple Cortical Areas SMART-seq

This data set includes single-nucleus transcriptomes from 49,495 nuclei across multiple human cortical areas. Individual layers of cortex were dissected from tissues covering the middle temporal gyrus (MTG), anterior cingulate cortex (ACC; also known as the ventral division of medial prefrontal cortex, A24), primary visual cortex (V1C), primary motor cortex (M1C), primary somatosensory cortex (S1C) and primary auditory cortex (A1C) derived from human brain. Nuclei were dissociated and sorted using the neuronal marker NeuN. Nuclei were sampled from post-mortem and neurosurgical (MTG only) donor brains and expression was profiled with SMART-Seq v4 RNA-sequencing.

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General

File

Size

Type

Description

12 KB

.txt

Read-me file with additional information about the other files in this directory.

13 MB

.csv

Metadata about cells. Each entry has an _order, _label, and _color column.

2.1 MB

.csv

TSNE coordinates for each cell, as shown in the Transcriptomics Explorer.

260 KB

.csv

Maps new sample ids (in metadata above) to sample ids released in October 2019.

Gene Expression

File

Size

Type

Description

5.0 GB

.csv

Gene expression matrices provided as a csv. Exon and intron counts are added together in this one csv. See "readme.txt" for data access instructions.

5.0 GB

.csv

Gene expression matrices provided as a csv. Intron counts only.

5.0 GB

.csv

Gene expression matrices provided as a csv. Exon counts only.

32 MB

.csv

Gene expression aggregated per cell type, calculated as trimmed means.

28 MB

.csv

Gene expression aggregated per cell type, calculated as medians.

Cell Types Taxonomy

File

Size

Type

Description

280 KB

.json

Serialized cell type hierarchy with all node information embedded.

1 KB

.txt

Metadata about the taxonomy.