Reference Genome Files (.gtf)

This page includes General Transfer Format (.gtf) or related files which allow one to reproduce the alignment from raw (fastq) sequencing data to a specific version of the genome/ transcriptome in RNA-seq analysis. The top table shows a data type-centric view of these files, while the bottom table shows at project-centric view of the same files. In both tables links are colored in blue for convenience.

Most individuals wanting to use processed versions of Allen Institute sequencing data (e.g., cell x gene matrices) can safely ignore this page.

In the tables below, 'SSv4' indicates any single cell or single nucleus RNA-sequencing or Patch-Seq data that was processed using the SMARTerV4 method, while '10x' indicates any single cell or single nucleus RNA-sequencing data processed using 10x Genomics single-omics or multiomics methods. All mouse and human single cell or single nucleus RNA-sequencing data was aligned to some version of the GRCm38 and GRCh38 genomics, respectively, although file formats and transcriptomics versions differ between rows. NA in the table means no data has every been generated in the relevant slot, while "in process" means that data has been generated but currently is not included in any Allen Brain Map tools. Finally, data from several projects with data generated prior to 2022 (including Patch-seq data) were originally processed using one transcriptome version and have since been converted into the current version. In this case, refer to the relevant Allen Brain Map or scientific public to determine which genome/transcriptome version was used. Abbreviations: GBM = Ivy Glioblastoma Atlas Project; TBI = Aging, Dementia and Traumatic Brain Injury Study; WHB = Whole human brain atlas included in ABC Atlas (May 2024); CTKE = Cell Type Knowledge Explorer; LGN = Lateral Geniculate Nucleus (part of Comparative LGN project)

Reference files by category and species

Data sets used

Human

Mouse

Marmoset

Macaque

Other mammals

10x processed using CellRanger V6 (~2022 - current)

in process

in process

in process

SSv4 (~2022 - current; conversion date same as 10x)

in process

in process

in process

10x processed using CellRanger V3 (start - ~2021)

NA

NA

NA

SSv4 (start - ~2021; conversion date same as 10x)

NA

NA

Projects with data processed by collaborators

NA

NA

NA

Historical bulk RNA-Seq data sets

NA

NA

NA

NA

Reference files by data set

Project Name

Species

Year

Modality

Take me to the project!

Reference file

Seattle Alzheimer’s Disease Brain Cell Atlas

Human

Ongoing

10x

Human Patch-seq

Human

Ongoing

SSv4

GRCh38/gencode.v32 (data in some manuscripts use GRCh38.p2)

Mouse Patch-seq

Mouse

Ongoing

SSv4

mm10/genecode.vM23 (data in some manuscripts use GRCm38.p3)

Human Whole Brain Atlas

Human

2023

10x

Mouse Whole Brain Atlas

Mouse

2023

10x

MTG - 10x SEA-AD (2022)

Human

2022

10x

Marmoset - M1 (CTKE)

Marmoset

2021

10x

Mouse - M1 (CTKE)

Mouse

2021

10x

M1 - 10x genomics (2020) (Transcriptomics Explorer and CTKE)

Human

2020

10x

Whole Cortex & Hippocampus - 10x genomics (2020)

Mouse

2020

10x

Whole Cortex & Hippocampus - SMART-seq (2019)

Mouse

2019

SSv4

Multiple Cortical Areas - SMART-seq (2019)

Human

2019

SSv4

MTG - SMART-seq (2018)

Human

2018

SSv4

V1 & ACC - SMART-seq (2018)

Human

2018

SSv4

VISp & ALM - SMART-seq (2018)

Mouse

2018

SSv4

ACA & MOp - SMART-seq (2018)

Mouse

2018

SSv4

Human - LGN (2018)

Human

2018

SSv4

Macaque - LGN (2018)

Macaque

2018

SSv4

Mouse - LGN (2018)

Mouse

2018

SSv4

Aging, Dementia, and TBI Study

Human

2017

Other

Ivy Glioblastoma Atlas Project

Human

2015

Other

BrainSpan Atlas of the Developing Human Brain

Human

2014

Other